Progesteron Testing


We offer a swab testing to check when the cells are changing and when the bitch is ready to be mated. This method is only good for bitches been mated to a live dog or fresh semen and does not replace the most accucate of testing by taking blood but can be very helpful and affordable in your breeding program.


Stages of the Estrous Cycle 


Proestrus:Vulvar swelling and bloody vaginal discharge marks the beginning of proestrus. The first day of bleeding is called the first day of heat. The duration of proestrus can vary from as little as 1 day to 21 days. Proestrus is a time of rising estrogen levels preparing the reproductive tract for breeding. 


Estrus:Is the period of receptivity. Behaviorally it begins the first day the bitch stands. Hormonally it begins on the day of the LH surge and is marked by rising progesterone levels and decreasing estrogen levels. 


Diestrus:Normally begins 7 to 9 days post the actual LH surge. Progesterone levels continue to rise and the vaginal cytology cornification abruptly declines. The diestrus period lasts until the bitch whelps or the serum progesterone returns to a basal level. 


Anestrus:Is the period of time between the end of diestrus and the next proestrus period. 



The vaginal mucosa becomes edematous as a result of high estrogen levels in proestrus. On vaginoscopy the vaginal wall has a billowing pillow appearance. Close to the LH surge the decreasing serum estrogen level and rising progesterone level causes a decrease in vaginal edema. The loss off edema in the previously swollen vaginal folds results in a wrinkled or crenulated appearance of the surface. Maximal crenulation or angulation of the folds occurs a few days later corresponding to the optimum fertile period. As diestrus approaches, the vaginal mucosa takes on a blotchy white and pink appearance. Vaginoscopy is a very useful tool in estimating the LH surge. 

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